Cobalt-containing calcium phosphate induces resorption of biomineralized collagen by human osteoclasts
نویسندگان
چکیده
Abstract Background Biomineralized collagen, consisting of fibrillar type-I collagen with embedded hydroxyapatite mineral, is a bone-mimicking material potential application as bone graft substitute. Despite the chemical and structural similarity extracellular matrix, no evidence exists so far that biomineralized can be resorbed by osteoclasts. The aim current study was to induce resorption osteoclasts two-fold modification: increasing calcium phosphate content introducing cobalt ions (Co 2+ ), which have been previously shown stimulate resorptive activity Methods To this end, we produced membranes coated them cobalt-containing (CoCaP). Human osteoclasts, derived from CD14+ monocytes peripheral blood, were differentiated directly on membranes. Upon fluorescent staining nuclei, F-actin tartrate-resistant alkaline phosphatase, cells analyzed laser confocal microscopy. Their capacity assessed scanning electron microscopy (SEM), well indirectly quantified measuring release into cell culture medium. Results CoCaP coating increased mineral 4 wt.% their elastic modulus 1 10 MPa. showed sustained Co in water about 7 nM per 2 days. In contrast uncoated membranes, CoCaP-coated sporadically formed actin rings, induced formation lacunae, observed SEM confirmed increase Ca concentration effect layer osteoclast function thought mainly caused membrane stiffness, although , released very low amounts, cannot fully excluded. Conclusions This work shows relatively simple approach extent limited, method needs further optimization. Moreover, suitable for incorporating bioactive interest typically not possible using common biomineralization methods, such polymer-induced liquid precursor method.
منابع مشابه
Preparation of mineralized nanofibers: collagen fibrils containing calcium phosphate.
We report a straightforward, bottom-up, scalable process for preparing mineralized nanofibers. Our procedure is based on flowing feed solution, containing both inorganic cations and polymeric molecules, through a nanoporous membrane into a receiver solution with anions, which leads to the formation of mineralized nanofibers at the exit of the pores. With this strategy, we were able to achieve s...
متن کاملRegulation and enzymatic basis of bone resorption by human osteoclasts.
Although much has been learned recently of the mechanisms that regulate osteoclastic differentiation, much less is known of the means through which their resorptive activity is controlled. This is especially so for human osteoclasts. We have recently developed an assay that allows us to measure resorptive activity while minimizing confounding effects on differentiation by optimizing osteoclasto...
متن کاملResorption of bone by isolated rabbit osteoclasts.
A carborundum wheel was used to prepare slices of cortical bone that demonstrate a predictable surface appearance in the scanning electron microscope. Osteoclasts were mechanically disaggregated from neonatal rabbit long bones and settled onto these slices. After 24h in culture osteoclasts were associated with areas of excavation in the bone surface. These excavated areas typically showed a wel...
متن کاملSpectrophotometric determination of Dalacin phosphate from formulations using reduce atomic absorption of calcium ion by phosphate
A simple, sensitive and accurate method for spectrophotometric determination of Dalacinphosphatehas been established. The method is based on the reduction of atomic absorptioncalcium ion by phosphate. Because, there is a phosphate mole in every Dalacin mole structureand with measurement of phosphate, Dalacin is beyond measurement too. Therefore, the reactionbetween acidic hydrolysis products of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomaterials Research
سال: 2021
ISSN: ['2055-7124', '1226-4601']
DOI: https://doi.org/10.1186/s40824-021-00209-7